Biochemical characterization of Arabidopsis APYRASE family reveals their roles in regulating endomembrane NDP/NMP homoeostasis
Tsan-Yu Chiu, Jeemeng Lao, Bianca Manalansan, Dominique Loque, Stanley J Roux, Joshua L Heazlewood
BIOCHEMICAL JOURNAL | PORTLAND PRESS LTD | Published : 2015
Plant apyrases are nucleoside triphosphate (NTP) diphosphohydrolases (NTPDases) and have been implicated in an array of functions within the plant including the regulation of extracellular ATP. Arabidopsis encodes a family of seven membrane bound apyrases (AtAPY1-7) that comprise three distinct clades, all of which contain the five conserved apyrase domains. With the exception of AtAPY1 and AtAPY2, the biochemical and the sub-cellular characterization of the other members are currently unavailable. In this research, we have shown all seven Arabidopsis apyrases localize to internal membranes comprising the cis-Golgi, endoplasmic reticulum (ER) and endosome, indicating an endo-apyrase classifi..View full abstract
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Awarded by Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy
Awarded by Australian Research Council
This work was supported by the Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy [grant number DE-AC02-05CH11231]; and the Australian Research Council Future Fellowship [grant number JLH FT130101165].