Journal article
Differential regulation of clathrin and its adaptor proteins during membrane recruitment for endocytosis
C Wang, T Hu, X Yan, T Meng, Y Wang, Q Wang, X Zhang, Y Gu, C Sánchez-Rodríguez, A Gadeyne, J Lin, S Persson, D van Damme, C Li, SY Bednarek, J Pan
Plant Physiology | Published : 2016
DOI: 10.1104/pp.15.01716
Abstract
In plants, clathrin-mediated endocytosis (CME) is dependent on the function of clathrin and its accessory heterooligomeric adaptor protein complexes, ADAPTOR PROTEIN2 (AP-2) and the TPLATE complex (TPC), and is negatively regulated by the hormones auxin and salicylic acid (SA). The details for how clathrin and its adaptor complexes are recruited to the plasma membrane (PM) to regulate CME, however, are poorly understood. We found that SA and the pharmacological CME inhibitor tyrphostin A23 reduce the membrane association of clathrin and AP-2, but not that of the TPC, whereas auxin solely affected clathrin membrane association, in Arabidopsis (Arabidopsis thaliana). Genetic and pharmacologica..
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Awarded by National Science Foundation
Funding Acknowledgements
This work was supported by the National Natural Science Foundation of China (grant nos. 91317304, 31370313, and 31171520), by a Vilas Associate Award from the University of Wisconsin, Madison, Graduate School (to S.Y.B.), and by the National Science Foundation (grant no. 1121998 to S.Y.B.).