Journal article
The circadian oscillator gene GIGANTEA mediates a long-term response of the Arabidopsis thaliana circadian clock to sucrose
N Dalchau, SJ Baek, HM Briggs, FC Robertson, AN Dodd, MJ Gardner, MA Stancombe, MJ Haydon, GB Stan, JM Gonçalves, AAR Webb
Proceedings of the National Academy of Sciences of the United States of America | Published : 2011
Abstract
Circadian clocks are 24-h timing devices that phase cellular responses; coordinate growth, physiology, and metabolism; and anticipate the day-night cycle. Here we report sensitivity of the Arabidopsis thaliana circadian oscillator to sucrose, providing evidence that plant metabolism can regulate circadian function. We found that the Arabidopsis circadian system is particularly sensitive to sucrose in the dark. These data suggest that there is a feedback between the molecular components that comprise the circadian oscillator and plant metabolism, with the circadian clock both regulating and being regulated by metabolism. We used also simulations within a three-loop mathematical model of the A..
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Awarded by Engineering and Physical Sciences Research Council
Funding Acknowledgements
We thank Prof. G. Coupland for donation of the Wassilewskija ecotype gi-11 CAB2::luc seed line. We thank the Biotechnology and Biological Sciences Research Council, United Kingdom, for grants to A. A. R. W. (supporting N.D., A.N.D., H. M. B., F. C. R., M. A. S., and M.J.H.) and the Engineering and Physical Sciences Research Council, United Kingdom, for a grant to A. A. R. W. and J.M.G. (supporting G.-B.S.). G.-B.S. also acknowledges the support of the Engineering and Physical Sciences Research Council-funded Centre for Synthetic Biology and Innovation at Imperial College London where he did part of this work. S.J.B. is grateful for funding from the Korean Science Foundation. M.J.G. was a Corpus Christi College, Cambridge Junior Research Fellow and is also grateful to the Royal Society of London for a grant supporting his research. A. A. R. W. acknowledges the financial support of the Isaac Newton Trust, Cambridge, allowing the purchase of a photon-counting camera used in this study.