Cutting Edge: A Thymocyte-Thymic Epithelial Cell Cross-Talk Dynamically Regulates Intrathymic IL-7 Expression In Vivo
Nuno L Alves, Nicholas D Huntington, Jean-Jacques Mention, Odile Richard-Le Goff, James P Di Santo
The Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2010
Thymic epithelial cells (TECs) are the predominant intrathymic source of the essential thymopoietin IL-7. Whether thymocyte-TEC interactions have a role in the regulation of IL-7 expression is not known. By exploiting IL-7 reporter mice in which yellow fluorescent protein expression identifies TECs expressing high levels of IL-7 (Il7(+) TECs), we show that Il7(+) TECs segregate from emerging medullary TECs during thymic organogenesis. Although Il7(+) TECs normally diminish with age, we found that Il7(+) TECs are markedly retained in alymphoid Rag2(-/-)Il2rg(-/-) IL-7 reporter mice that manifest a profound thymopoietic arrest. Transfer of Tcra(-/-) or wild-type (but not Rag2(-/-)) hematopoiet..View full abstract
Awarded by Fondation pour le Recherche Medicale
Awarded by Foundation for Science and Technology
This work was supported in part by Institut Pasteur, Institut National de la Sante et de la Recherche Medicale, the Fondation pour le Recherche Medicale (SPF20081214865), the Ligue Nationale Contre le Cancer, and the Foundation for Science and Technology (SFRH/45932/2008 and Program Ciencia 2008).