Journal article
The 60- to 90-kDa parietal cell autoantigen associated with autoimmune gastritis is a β subunit of the gastric H /K -ATPase (proton pump)
BH Toh, PA Gleeson, RJ Simpson, RL Moritz, JM Callaghan, I Goldkorn, CM Jones, TM Martinelli, FT Mu, DC Humphris, JM Pettitt, Y Mori, T Masuda, P Sobieszczuk, J Weinstock, T Mantamadiotis, GS Baldwin
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 1990
Abstract
Autoantibodies in the sera of patients with pernicious anemia recognize, in addition to the α subunit of the gastric H+/K+-ATPase, an abundant gastric microsomal glycoprotein of apparent Mr 60,000-90,000. Herein we have colocalized the glycoprotein and the α subunit of the gastric H+/K+-ATPase to the tubulovesicular membranes of the parietal cell by immunogold electron microscopy. Moreover, the glycoprotein and the α subunit were coimmunoprecipitated, and copurified by immunoaffinity chromatography, with an anti-glycoprotein monoclonal antibody. The pig glycoprotein was purified by chromatography on tomato lectin-Sepharose, and five tryptic peptides from the purified glycoprotein were partia..
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