Mouse Norovirus infection promotes autophagy induction to facilitate replication but prevents final autophagosome maturation
Tanya B O'Donnell, Jennifer L Hyde, Justine D Mintern, Jason M Mackenzie
VIROLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2016
Autophagy is a cellular process used to eliminate intracellular pathogens. Many viruses however are able to manipulate this cellular process for their own advantage. Here we demonstrate that Mouse Norovirus (MNV) infection induces autophagy but does not appear to utilise the autophagosomal membrane for establishment and formation of the viral replication complex. We have observed that MNV infection results in lipidation and recruitment of LC3 to the autophagosome membrane but prevents subsequent fusion of the autophagosomes with lysosomes, as SQSTM1 (an autophagy receptor) accumulates and Lysosome-Associated Membrane Protein1 is sequestered to the MNV replication complex (RC) rather than to ..View full abstract
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Awarded by National Health and Medical Research Council of Australia
This research was supported by a research grants to J.M.M. from the National Health and Medical Research Council of Australia (Grant nos. 1010327 and 1083139). We thank Dr Karla. Kirkegaard (Stanford University, USA) for the LC3-GFP plasmid. We also thank Herbert "Skip" Virgin and Kim Green for generously provided antibodies.