Eosinophil-Associated Gene Pathways but not Eosinophil Numbers are Differentially Regulated between Synchrotron Microbeam Radiation Treatment and Synchrotron Broad-Beam Treatment by 48 Hours Postirradiation
MJ Ibahim, Y Yang, JC Crosbie, A Stevenson, L Cann, P Paiva, PA Rogers
RADIATION RESEARCH | RADIATION RESEARCH SOC | Published : 2016
Synchrotron microbeam radiation treatment (MRT) is a preclinical radiotherapy technique with considerable clinical promise, although some of the underlying radiobiology of MRT is still not well understood. In recently reported studies, it has been suggested that MRT elicits a different tumor immune profile compared to broad-beam treatment (BB). The aim of this study was to investigate the effects of synchrotron MRT and BB on eosinophil-associated gene pathways and eosinophil numbers within and around the tumor in the acute stage, 48 h postirradiation. Balb/C mice were inoculated with EMT6.5 mouse mammary tumors and irradiated with microbeam radiation (112 and 560 Gy) and broad-beam radiation..View full abstract
This research was undertaken at the Imaging and Medical Beam Line (IMBL) at the Australian Synchrotron, Victoria, Australia. JCC and PP are NHMRC Early Career Research Fellows. MI acknowledges funding and sponsorship from the Malaysian Government. We acknowledge funding (2013 grant-in-aid) from Cancer Council Victoria. We thank Mr. Cameron Nowell, Monash Institute of Pharmaceutical Sciences, Monash University for assistance in developing the Fiji Macro.