Journal article
Nonesterified fatty acid-induced endoplasmic reticulum stress in cattle cumulus oocyte complexes alters cell metabolism and developmental competence
ML Sutton-McDowall, LLY Wu, M Purdey, AD Abell, EM Goldys, KL MacMillan, JG Thompson, RL Robker
Biology of Reproduction | Published : 2016
Abstract
Reduced oocyte quality has been associated with poor fertility of high-performance dairy cows during peak lactation, due to negative energy balance. We examined the role of nonesterified fatty acids (NEFAs), known to accumulate within follicular fluid during under- and overnutrition scenarios, in causing endoplasmic reticulum (ER) stress of in vitro maturated cattle cumulusoocyte complexes (COCs). NEFA concentrations were: palmitic acid (150 μM), oleic acid (200 μM), and steric acid (75 μM). Abattoir-derived COCs were randomly matured for 24 h in the presence of NEFAs and/or an ER stress inhibitor, salubrinal. Total and hatched blastocyst yields were negatively impacted by NEFA treatment com..
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Awarded by Gardiner Foundation
Awarded by Australian Research Council Centre of Excellence for Nanoscale BioPhotonics
Awarded by National Health & Medical Research Council
Awarded by National Health and Medical Research Council of Australia
Funding Acknowledgements
This study was funded by an innovation grant from the Gardiner Foundation (INN-12-041). The Fluoview FV10i confocal microscope was purchased as part of the Sensing Technologies for Advanced Reproductive Research (STARR) facility, funded by the South Australia's Premier's Science and Research Fund. We acknowledge partial support of the Australian Research Council Centre of Excellence for Nanoscale BioPhotonics (CE140100003). J.G.T. is supported by a National Health & Medical Research Council Research Fellowship (ID 1077694). R.L.R. is supported by a National Health & Medical Research Council Research RD Wright Career Development Fellowship (ID 1044969).