Journal article
Novel Elements of the Chondrocyte Stress Response Identified Using an in Vitro Model of Mouse Cartilage Degradation
R Wilson, SB Golub, L Rowley, C Angelucci, YV Karpievitch, JF Bateman, AJ Fosang
Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2016
Abstract
The destruction of articular cartilage in osteoarthritis involves chondrocyte dysfunction and imbalanced extracellular matrix (ECM) homeostasis. Pro-inflammatory cytokines such as interleukin-1α (IL-1α) contribute to osteoarthritis pathophysiology, but the effects of IL-1α on chondrocytes within their tissue microenvironment have not been fully evaluated. To redress this we used label-free quantitative proteomics to analyze the chondrocyte response to IL-1α within a native cartilage ECM. Mouse femoral heads were cultured with and without IL-1α, and both the tissue proteome and proteins released into the media were analyzed. New elements of the chondrocyte response to IL-1α related to cellula..
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Awarded by National Health and Medical Research Council of Australia
Awarded by Australian Research Council
Funding Acknowledgements
This work was funded in part by grants from the National Health and Medical Research Council of Australia (#1063133, #1002797, #491237). We acknowledge the use of equipment and infrastructure provided by the Central Science Laboratory, University of Tasmania, funded by Australian Research Council grant LE0775570.