Journal article
MicroRNAs in CD4 T cell subsets are markers of disease risk and T cell dysfunction in individuals at risk for type 1 diabetes
Y Zhang, ZP Feng, G Naselli, F Bell, J Wettenhall, P Auyeung, JA Ellis, AL Ponsonby, TP Speed, MMW Chong, LC Harrison
Journal of Autoimmunity | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2015
Abstract
© 2016 Elsevier Ltd. MicroRNAs (miRNAs) regulate T cell development and function and the disruption of miRNAs in natural regulatory CD4+ FOXP3+ T cells (nTreg) leads to autoimmune disease in mice. To investigate miRNA expression in relation to autoimmune disease risk in humans we sequenced them in purified CD4+ T cell subsets from individuals at high risk of type 1 diabetes (pre-T1D), as well as other healthy individuals. Differences in miRNA expression patterns were observed between specific T cell subsets and, within subsets, between pre-T1D and healthy individuals. Compared to healthy, naive CD4+ T cells in pre-T1D displayed 32 differentially expressed miRNAs, potentially a template for a..
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Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by the Juvenile Diabetes Research Foundation (JDRF) (Project Grants 5-2011-100 to M.M.W.C. and 17-2013-547 to L.C.H.), the Australian National Health and Medical Research Council (NHMRC) (Project Grants 637338 and 1004541 to M.M.W.C., Program Grant 1037321 to L.C.H. and Project Grant 1026349 to J.A.E and A.L.P), the Rebecca Cooper Medical Research Foundation (to M.M.W.C.) and Walter and Eliza Hall Institute Genomics Fund (to Y.Z). Y.Z. was a JDRF Post-doctoral Fellow (3-2010-448). J.A.E was a Future Fellow and M.M.W.C. a QEII Fellow of the Australian Research Council. A.L.P. was a Senior Research Fellow (1008396), L.C.H. a Senior Principal Research Fellow (637301) and T.P.S. an Australia Fellow (575503) of the NHMRC. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian National Health and Medical Research Council Research Institute Infrastructure Support Scheme.