Chondrogenesis of Human Infrapatellar Fat Pad Stem Cells on Acellular Dermal Matrix

K Ye; K Traianedes; PFM Choong; DE Myers

Journal article

Chondrogenesis of Human Infrapatellar Fat Pad Stem Cells on Acellular Dermal Matrix

K Ye, K Traianedes, PFM Choong, DE Myers

Frontiers in Surgery | Published : 2016

DOI: 10.3389/fsurg.2016.00003

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Abstract

Acellular dermal matrix (ADM) has been in clinical use for decades in numerous surgical applications. The ability for ADM to promote cellular repopulation, revascularisation and tissue regeneration is well documented. Adipose stem cells have the ability to differentiate into mesenchymal tissue types, including bone and cartilage. The aim of this study was to investigate the potential interaction between ADM and adipose stem cells in vitro using TGFβ3 and BMP6. Human infrapatellar fat pad-derived adipose stem cells (IPFP-ASC) were cultured with ADM derived from rat dermis in chondrogenic (TGFβ3 and BMP6) medium in vitro for 2 and 4 weeks. Histology, qPCR, and immunohistochemistry were perform..

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University of Melbourne Researchers

Damian Myers Author

Peter Choong Author

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Grants

Awarded by National Science Foundation

Funding Acknowledgements

This work was funded through the Australian Orthopaedics Association Research Foundation (AOA Research Foundation) and National Health and Medical Research Council (NHMRC) Postgraduate Scholarship for author KY (Grant number APP1017633).