Journal article

Temporal changes of antibiotic-resistance genes and bacterial communities in two contrasting soils treated with cattle manure

Hang-Wei Hu, Xue-Mei Han, Xiu-Zhen Shi, Jun-Tao Wang, Li-Li Han, Deli Chen, Ji-Zheng He

FEMS MICROBIOLOGY ECOLOGY | OXFORD UNIV PRESS | Published : 2016

Abstract

The emerging environmental spread of antibiotic-resistance genes (ARGs) and their subsequent acquisition by clinically relevant microorganisms is a major threat to public health. Animal manure has been recognized as an important reservoir of ARGs; however, the dissemination of manure-derived ARGs and the impacts of manure application on the soil resistome remain obscure. Here, we conducted a microcosm study to assess the temporal succession of total bacteria and a broad spectrum of ARGs in two contrasting soils following manure application from cattle that had not been treated with antibiotics. High-capacity quantitative PCR detected 52 unique ARGs across all the samples, with β-lactamase as..

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