Journal article

Evaluation of an IgG enzyme-linked immunosorbent assay as a serological assay for detection of mycoplasma bovis infection in feedlot cattle

NK Wawegama, PF Markham, A Kanci, M Schibrowski, S Oswin, TS Barnes, SM Firestone, TJ Mahony, GF Browning

Journal of Clinical Microbiology | American Society for Microbiology | Published : 2016


Copyright © 2016, American Society for Microbiology. All Rights Reserved. Mycoplasma bovis is a pathogen of emerging significance in cattle throughout the world that is causing a range of diseases, including mastitis, arthritis, and pneumonia. The limited availability and efficacy of current diagnostic and prophylactic tools for its control and its increasing antimicrobial resistance are contributing to its increasing importance in beef and dairy cattle. We have developed an indirect IgG enzyme-linked immunosorbent assay (ELISA) based on a recombinant fragment of the MilA protein and have shown its potential as an effective diagnostic tool. To more comprehensively estimate the diagnostic sen..

View full abstract


Awarded by Australian Research Council

Awarded by Meat and Livestock Australia

Funding Acknowledgements

This work, including the efforts of Anna Kanci, Sally Oswin, Timothy John Mahony, Glenn F. Browning, Nadeeka Kumari Wawegama, and Philip Francis Markham, was funded by Zoetis Australia Pty. Ltd. This work, including the efforts of Nadeeka Kumari Wawegama, Philip Francis Markham, Anna Kanci, and Glenn F. Browning, was funded by Australian Research Council (LP0989620). This work, including the efforts of Meghan Schibrowski, Tamsin S. Barnes, and Timothy John Mahony, was funded by Meat and Livestock Australia (B.FLT.0224).