Journal article

Spheres of influence: Porphyromonas gingivalis outer membrane vesicles

MJ Gui, SG Dashper, N Slakeski, Y-Y Chen, EC Reynolds

MOLECULAR ORAL MICROBIOLOGY | WILEY | Published : 2016

Abstract

Outer membrane vesicles (OMVs) are asymmetrical single bilayer membranous nanostructures produced by Gram-negative bacteria important for bacterial interaction with the environment. Porphyromonas gingivalis, a keystone pathogen associated with chronic periodontitis, produces OMVs that act as a virulence factor secretion system contributing to its pathogenicity. Despite their biological importance, the mechanisms of OMV biogenesis have not been fully elucidated. The ~14 times more curvature of the OMV membrane than cell outer membrane (OM) indicates that OMV biogenesis requires energy expenditure for significant curvature of the OMV membrane. In P. gingivalis, we propose that this may be achi..

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Grants

Funding Acknowledgements

We are grateful for the technical assistance of Mrs Caroline Moore in the preparation of cells and OMVs for microscopic imaging. Dr. Simon Crawford for the scanning electron microscopy to visualize P. gingivalis W50 cells is gratefully acknowledged. Chris Owen is thanked for constructing a figure demonstrating P. gingivalis OMV biogenesis and their putative functions. Gui May Ju is supported by International Postgraduate Research Scholarship funded by the Australian Government under the University of Melbourne.