Journal article

Induction of memory cytotoxic T cells to influenza A virus and subsequent viral clearance is not modulated by PB1-F2-dependent inflammasome activation

Patricia Hoi Yee Lee, Nicola Bird, Charley MacKenzie-Kludas, Ashley Mansell, Katherine Kedzierska, Lorena Brown, Julie McAuley

IMMUNOLOGY AND CELL BIOLOGY | NATURE PUBLISHING GROUP | Published : 2016

Abstract

Expression of the viral virulence protein PB1-F2 during infection has been linked to NLRP3 inflammasome complex activation in macrophages and induction of early inflammatory events enhancing immunopathology during influenza disease. We sought to determine whether PB1-F2-specific NLRP3 inflammasome activation influenced the magnitude and/or robustness of the CD8(+) T-cell responses specific for conserved viral antigens and subsequent virus elimination. Using murine heterosubtypic viral infection models, we showed that mice infected with virus unable to produce PB1-F2 protein showed no deficit in the overall magnitude and functional memory responses of CD8(+) T cells established during the eff..

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Grants

Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by NHMRC


Funding Acknowledgements

JM is funded by the National Health and Medical Research Council of Australia (NHMRC) project grants 1026619 and 1079924; KK is a CDF2 NHMRC Fellow; KK and LB receive funding from NHMRC program grant 1071916. The authors acknowledge St Jude Children's Research Hospital (TN, USA) as the original source of the PR8 PB1 and PR8 Delta PB1-F2 plasmids.