Journal article
BCL2-modifying factor promotes germ cell loss during murine oogenesis
K Vaithiyanathan, SH Liew, N Zerafa, T Gamage, M Cook, LA O'Reilly, P Bouillet, CL Scott, A Strasser, JK Findlay, KJ Hutt
Reproduction | Published : 2016
DOI: 10.1530/REP-15-0561
Abstract
Apoptosis plays a prominent role during ovarian development by eliminating large numbers of germ cells from the female germ line. However, the precise mechanisms and regulatory proteins involved in germ cell death are yet to be determined. In this study, we characterised the role of the pro-apoptotic BH3-only protein, BCL2-modifying factor (BMF), in germ cell apoptosis in embryonic and neonatal mouse ovaries. BMF protein was immunohistochemically localised to germ cells at embryonic days 15.5 (E15.5) and E17.5 and postnatal day 1 (PN1), coincident with entry into the meiotic prophase, but was undetectable at E13.5, and only present at low levels at PN3 and PN5. Consistent with this expressio..
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Awarded by National Health and Medical Research Council (NHMRC Australia)
Awarded by National Health and Medical Research Council of Australia
Funding Acknowledgements
This work was supported by fellowships and grants from the National Health and Medical Research Council (NHMRC Australia; project grant, K J Hutt (no. 1007027), C L Scott (no. 1062702); program grant nos 494802 and 257502; fellowships, J K Findlay (no. 441101), K J Hutt (no. 1050130), C L Scott (no. 406675), P Bouillet (no. 1042629), A Strasser (no. 461299)), the Cancer Council Victoria (Sir Edward Dunlop Fellowship in Cancer Research, C L Scott), and the Victorian Cancer Agency (clinical fellowship, C L Scott). This work was made possible through the Australian Cancer Research Foundation, the Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS.