Journal article
Inductive interactions mediated by interplay of asymmetric signalling underlie development of adult haematopoietic stem cells
C Souilhol, C Gonneau, JG Lendinez, A Batsivari, S Rybtsov, H Wilson, L Morgado-Palacin, D Hills, S Taoudi, J Antonchuk, S Zhao, A Medvinsky
Nature Communications | Published : 2016
DOI: 10.1038/ncomms10784
Abstract
During embryonic development, adult haematopoietic stem cells (HSCs) emerge preferentially in the ventral domain of the aorta in the aorta-gonad-mesonephros (AGM) region. Several signalling pathways such as Notch, Wnt, Shh and RA are implicated in this process, yet how these interact to regulate the emergence of HSCs has not previously been described in mammals. Using a combination of ex vivo and in vivo approaches, we report here that stage-specific reciprocal dorso-ventral inductive interactions and lateral input from the urogenital ridges are required to drive HSC development in the aorta. Our study strongly suggests that these inductive interactions in the AGM region are mediated by the ..
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Awarded by Seventh Framework Programme
Funding Acknowledgements
We thank J. Verth, C. Manson and R. McInnis for assistance with mouse maintenance and breeding, C. Watt., C. Flockhart, C. Forrest and A. Dyer for irradiations, S. Monard, O. Rodriguez, F. Rossi and C. Cryer for cell sorting and B. Vernay for assistance with confocal microscopy. We thank S. Morrison for providing SCF-GFP reporter mice; V. Ribes and J. Briscoe for GBS-GFP reporter mice; H. Niwa for the plasmid pPBhCMV1-cHA-IRES-VenuspA and Dr Stephen Gould, Genentech for providing Shh antagonist HhAntag. We thank M. Cohen-Tannoudji, J. Davis, J. Easterbrook, A. McGarvey, M. Gehring, S. Gordon-Keylock, B. Gottgens, S. Lowell, J. Pimanda, A. Tsakiridis, L. Forrester, P. Travers and V. Wilson for helpful comments. This work was supported by LLR, BBSRC, MRC, Wellcome Trust and the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme FP7/2007-2013/under REA grant agreement no 317250.