Minimizing matrix effects for the accurate quantification of 25-hydroxyvitamin D metabolites in dried blood spots by LC-MS/MS

Abstract

BACKGROUND: The noncalcemic actions of Vitamin D in multiple organs are now widely recognized. Vitamin D status has been linked with a wide variety of conditions, which has led to an increasing demand for Vitamin D screening. In particular, there is intense interest in the impact of Vitamin D on a variety of developmental conditions. The most readily accessible pediatric samples are dried blood spots, and health organizations are increasingly archiving such samples for later assessment of the antecedents of disease. METHODS: In 2009, we developed a method to quantify the major circulatory form of Vitamin D, 25-hydroxyVitamin D, in archived dried blood spots. Over the last 6 years, we have ma..