Journal article

Linking the T cell receptor to the single cell transcriptome in antigen-specific human T cells

Auda A Eltahla, Simone Rizzetto, Mehdi R Pirozyan, Brigid D Betz-Stablein, Vanessa Venturi, Katherine Kedzierska, Andrew R Lloyd, Rowena A Bull, Fabio Luciani



Heterogeneity of T cells is a hallmark of a successful adaptive immune response, harnessing the vast diversity of antigen-specific T cells into a coordinated evolution of effector and memory outcomes. The T cell receptor (TCR) repertoire is highly diverse to account for the highly heterogeneous antigenic world. During the response to a virus multiple individual clones of antigen specific CD8+ (Ag-specific) T cells can be identified against a single epitope and multiple epitopes are recognised. Advances in single-cell technologies have provided the potential to study Ag-specific T cell heterogeneity at both surface phenotype and transcriptome levels, thereby allowing investigation of the dive..

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Awarded by NHMRC Practitioner Fellowship

Awarded by NHMRC Career Development Fellowship

Awarded by NHMRC SRFB fellowship

Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

We acknowledge NHMRC and ACH2 for funding. SR is supported by the University International Postgraduate Award UNSW Australia. ARL is supported by an NHMRC Practitioner Fellowship (No. 1043067), RAB is supported by an NHMRC Career Development Fellowship (No. 1060443), VV is supported by an NHMRC Career Development Fellowship (1067590), and KK is supported by an NHMRC SRFB fellowship (1102792). We are grateful to Simone Picelli for helpful clarifications on the SmartSeq2 protocol, and to Oanh Nguyen for technical assistance with the TCR-PCR protocol.