Elemental imaging of leaves from the metal hyperaccumulating plant Noccaea caerulescens shows different spatial distribution of Ni, Zn and Cd
Damien L Callahan, Dominic J Hare, David P Bishop, Philip A Doble, Ute Roessner
RSC ADVANCES | ROYAL SOC CHEMISTRY | Published : 2016
Elemental imaging using laser ablation inductively coupled plasma mass spectrometry was performed on whole leaves of the hyperaccumulating plant Noccaea caerulescens after treatments with either Ni, Zn or Cd. These detailed elemental images reveal differences in the spatial distribution of these three elements across the leaf and provide new insights in the metal ion homeostasis within hyperaccumulating plants. In the Zn treated plants, Zn accumulated in the leaf tip while Mn was co-localised with Zn suggesting similar storage mechanisms for these two metals. These data show a Zn concentration difference of up to 13-fold higher in the distal part of the leaf. Also, there was no correlation b..View full abstract
This research was supported by funding from Deakin University and the central research grants scheme (CRGS) and an early career researcher grant from The University of Melbourne. DL Callahan would like to thank Vasalisa Pendan for help with plant growth. The authors would also like to thank Prof. Alan Baker for provision of the N. caerulescens seed bank.