Detection of avian leukosis virus with the ELISA system: evaluation of conjugation methodology and comparison of sensitivity with the phenotypic mixing test in commercial layer flocks.
J Ignjatovic, TJ Bagust
Avian Pathology | Published : 1982
Conjugates of horseradish peroxidase with rabbit IgG antibody against gs antigen (p27) of avian myeloblastosis virus were prepared using glutaraldehyde and periodate methods of coupling. Conjugates were evaluated for the detection of gs antigen of avian leukosis and sarcoma viruses in the ELISA system and the periodate conjugate was found to be superior. With an ELISA based on a periodate conjugate it was possible to detect 5 x 10(3) IU of leukosis virus propagated in cell culture. Comparisons between the PM test and ELISA on biological samples showed the PM test to be 2 to 2,000 times more sensitive than ELISA for vaginal swabs and embryo extracts. ELISA was 16 to 64 times more sensitive th..View full abstract