Journal article

Human glioma-induced immunosuppression involves soluble factor(s) that alters monocyte cytokine profile and surface markers

JP Zou, LA Morford, C Chougnet, AR Dix, AG Brooks, N Torres, JD Shuman, JE Coligan, WH Brooks, TL Roszman, GM Shearer

Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 1999


Patients with gliomas exhibit deficient in vitro and in vivo T cell immune activity, and human glioblastoma culture supernatants (GCS) inhibit in vitro T lymphocyte responses. Because APC are essential for initiating and regulating T cell responses, we investigated whether GCS would affect cytokines produced by monocytes and T cells from healthy donors of PBMC. Incubation of PBMC with GCS decreased production of IL-12, IFN-gamma, and TNF-alpha, and increased production of IL-6 and IL-10. The GCS-induced changes in IL-12 and IL-10 occurred in monocytes, and involved changes in IL-12 p40 and IL-10 mRNA expression. Incubation with GCS also resulted in reduced expression of MHC class II and of C..

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University of Melbourne Researchers