Mapping nano-scale mechanical heterogeneity of primary plant cell walls

Grants

Funding Acknowledgements

This work was performed in part at the Queensland node of the Australian National Fabrication Facility (ANNF-Q), a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and microfabrication facilities for Australia's researchers. The authors thank Chee Meng (Tony) Chin (UM) for supply of the Lolium SCCs and polysaccharide analysis; Dr Kim L. Johnson (UM) for A. thaliana and L. multiflorum plant growth; Dr Sarah M. Wilson (UM) for TEM imaging of L. multiflorum SCCs; Dr Allison Van De Meene (UM) for SEM imaging of L. multiflorum SCCs; Dr Patricia Lopez-Sanchez (UQ) for protocols on cell viability assay and cell sieving; Dr Wei Zeng (UM) for preparation of plant materials; and Dr Sushil Dhital (UQ) for cryo-milling of SCC suspensions. HC and GY acknowledge Professor Justin Cooper-White (AIBN, UQ) for his support of this project. Professor Herman Hofte (INRA, France) and Professor Daniel Cosgrove (Penn State, USA) are gratefully acknowledged for many helpful discussions. MSD, AB, and MJG wish to acknowledge the support of the Australian Research Council for funding to the ARC Centre of Excellence in Plant Cell Walls (CE110001007).