Functional Analysis of a Bacitracin Resistance Determinant Located on ICECp1, a Novel Tn916-Like Element from a Conjugative Plasmid in Clostridium perfringens
Xiaoyan Han, Xiang-Dang Du, Luke Southey, Dieter M Bulach, Torsten Seemann, Xu-Xia Yan, Trudi L Bannam, Julian I Rood
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | AMER SOC MICROBIOLOGY | Published : 2015
Bacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. They act by interfering with the biosynthesis of the bacterial cell wall. In this study, we analyzed an avian necrotic enteritis strain of Clostridium perfringens that was resistant to bacitracin and produced NetB toxin. We identified a bacitracin resistance locus that resembled a bacitracin resistance determinant from Enterococcus faecalis. It contained the structural genes bcrABD and a putative regulatory gene, bcrR. Mutagenesis studies provided evidence that both bcrA and bcrB are essential for bacitracin resistance, and that evidence was supported by the results of experiments in which the in..View full abstract
Awarded by Australia Research Council (ARC)
This research was supported by grants from the Australia Research Council (ARC) to the ARC Centre of Excellence in Structural and Functional Microbial Genomics (grant CE0562063).