Journal article
Necrotic enteritis-derived clostridium perfringens strain with three closely related independently conjugative toxin and antibiotic resistance plasmids
TL Bannam, XX Yan, PF Harrison, T Seemann, AL Keyburn, C Stubenrauch, LH Weeramantri, JK Cheung, BA McClane, JD Boyce, RJ Moore, JI Rood
Mbio | Published : 2011
Open access
Abstract
The pathogenesis of avian necrotic enteritis involves NetB, a pore-forming toxin produced by virulent avian isolates of Clostridium perfringens type A. To determine the location and mobility of the netB structural gene, we examined a derivative of the tetracycline-resistant necrotic enteritis strain EHE-NE18, in which netB was insertionally inactivated by the chloramphen-icol and thiamphenicol resistance gene catP. Both tetracycline and thiamphenicol resistance could be transferred either together or separately to a recipient strain in plate matings. The separate transconjugants could act as donors in subsequent matings, which demonstrated that the tetracycline resistance determinant and the..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This research was supported by grants by the Australian Research Council to the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, and by grant 5R01AI056177-08 from the U.S. National Institute of Allergy and Infectious Diseases. This research was partly conducted within the Poultry CRC, established and supported under the Australian Government's Cooperative Research Centers Program. Xu-Xia Yan was the recipient of an Australian Postgraduate Scholarship.