Journal article

Genome Sequences and Phylogenetic Analysis of K88-and F18-Positive Porcine Enterotoxigenic Escherichia coli

Sara M Shepard, Jessica L Danzeisen, Richard E Isaacson, Torsten Seemann, Mark Achtman, Timothy J Johnson

Journal of bacteriology | AMER SOC MICROBIOLOGY | Published : 2012

Abstract

Porcine enterotoxigenic Escherichia coli (ETEC) continues to result in major morbidity and mortality in the swine industry via postweaning diarrhea. The key virulence factors of ETEC strains, their serotypes, and their fimbrial components have been well studied. However, most studies to date have focused on plasmid-encoded traits related to colonization and toxin production, and the chromosomal backgrounds of these strains have been largely understudied. Here, we generated the genomic sequences of K88-positive and F18-positive porcine ETEC strains and examined the phylogenetic distribution of clinical porcine ETEC strains and their plasmid-associated genetic content. The genomes of porcine E..

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University of Melbourne Researchers

Grants

Awarded by Science Foundation of Ireland


Funding Acknowledgements

We thank the University of Minnesota Veterinary Diagnostic Laboratory and Simone Oliveira for providing strains for this study. This work was carried out in part using computing resources at the University of Minnesota Supercomputing Institute. This project was funded in part by the Minnesota Pork Board and by Science Foundation of Ireland grant 05/FE1/B882. We gratefully acknowledge the curation of the MLST database by Vimalkumar Velayudhan and serotyping of strains by Chitrita Debroy at the Penn State University E. coli Reference Center.