Journal article

Collecting Duct-Derived Cells Display Mesenchymal Stem Cell Properties and Retain Selective In Vitro and In Vivo Epithelial Capacity

Joan Li, Usukhbayar Ariunbold, Norseha Suhaimi, Nana Sunn, Jinjin Guo, Jill A McMahon, Andrew P McMahon, Melissa Little



We previously described a mesenchymal stem cell (MSC)-like population within the adult mouse kidney that displays long-term colony-forming efficiency, clonogenicity, immunosuppression, and panmesodermal potential. Although phenotypically similar to bone marrow (BM)-MSCs, kidney MSC-like cells display a distinct expression profile. FACS sorting from Hoxb7/enhanced green fluorescent protein (GFP) mice identified the collecting duct as a source of kidney MSC-like cells, with these cells undergoing an epithelial-to-mesenchymal transition to form clonogenic, long-term, self-renewing MSC-like cells. Notably, after extensive passage, kidney MSC-like cells selectively integrated into the aquaporin 2..

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Awarded by National Health and Medical Research Council

Awarded by National Institutes of Health

Awarded by Center for Regenerative Medicine and Stem Cell Research Grant

Funding Acknowledgements

We thank N. Martel for his assistance with timed mating and animal maintenance, T. Hitchcock and her staff at the University of Queensland Biological Resources facility for their assistance with animal models, V. Nink and Dr. G. Osborne at the Flow Cytometry Facility, Queensland Brain Institute, for FACS sorting, and the staff at the Australian Cancer Research Foundation Imaging Facility for microscopy support. M.L. is a Senior Principal Research Fellow of the National Health and Medical Research Council of Australia. This work was funded by the Australian Stem Cell Centre, National Health and Medical Research Council Grant APP1054895, National Institutes of Health Grant DK054364 (to A.P.M.), and Center for Regenerative Medicine and Stem Cell Research Grant C10-06536 (to A.P.M.).