Journal article
Erythroid-extrinsic regulation of normal erythropoiesis by retinoic acid receptors
SR Dewamitta, C Joseph, LE Purton, CR Walkley
British Journal of Haematology | Published : 2014
DOI: 10.1111/bjh.12578
Abstract
Vitamin A and its derivatives (retinoids) are important regulators of haematopoiesis, acting via retinoic acid receptors (RARs). Epidemiological studies indicated an association of vitamin A deficiency with anaemia in humans. To define the requirements of RARs in erythropoiesis, we evaluated erythroid parameters in RAR germ-line deficient and conditional knock out mice with erythroid specific deletion of RARs. Adult RARγ-/- mice were anaemic, however, Epor-Cre Rarafl/fl, Epor-Cre Rargfl/fl and Epor-Cre Rarafl/flgfl/fl mice were normal, indicating a lack of an erythroid intrinsic RAR function. Therefore, erythroid-specific RAR function is dispensable for erythropoiesis and RARγ plays an eryth..
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Funding Acknowledgements
The authors would like to thank M. Wall and V Sankaran for comments and discussion; P. Chambon for generously providing RAR mouse lines; SVH BioResources Centre for care of experimental animals; B Liddicoat for technical assistance. This work was supported by grants from the Leukaemia Foundation, National Health and Medical Research Council of Australia (NHMRC; to C.W. and L.P.); Cancer Council Victoria (L.P.); NHMRC Career Development Award (C.W.); NHMRC Senior Research Fellowship (L.P.); in part by the Victorian State Government Operational Infrastructure Support Program (to St. Vincent's Institute). C.W. is the Philip Desbrow Senior Research Fellow of the Leukaemia Foundation.