Genomic imprinting of Dopa decarboxylase in heart and reciprocal allelic expression with neighboring Grb10.
Trevelyan R Menheniott, Kathryn Woodfine, Reiner Schulz, Andrew J Wood, David Monk, Andrew S Giraud, H Scott Baldwin, Gudrun E Moore, Rebecca J Oakey
Molecular and Cellular Biology | Published : 2008
By combining a tissue-specific microarray screen with mouse uniparental duplications, we have identified a novel imprinted gene, Dopa decarboxylase (Ddc), on chromosome 11. Ddc_exon1a is a 2-kb transcript variant that initiates from an alternative first exon in intron 1 of the canonical Ddc transcript and is paternally expressed in trabecular cardiomyocytes of the embryonic and neonatal heart. Ddc displays tight conserved linkage with the maternally expressed and methylated Grb10 gene, suggesting that these reciprocally imprinted genes may be coordinately regulated. In Dnmt3L mutant embryos that lack maternal germ line methylation imprints, we show that Ddc is overexpressed and Grb10 is sile..View full abstract
Awarded by Wellcome Trust