Journal article

MAPPING OF THE T-CELL AND B-CELL EPITOPES OF THE MYCOBACTERIUM-BOVIS PROTEIN, MPB70

H BILLMANJACOBE, AJ RADFORD, JS ROTHEL, PR WOOD

IMMUNOLOGY AND CELL BIOLOGY | BLACKWELL SCIENCE | Published : 1990

Abstract

A clone coding for the entire gene for the Mycobacterium bovis protein antigen MPB70 was used to produce a series of overlapping subclones by making a series of deletions from the 3' end of the gene. The subclones expressed incomplete MPB70 proteins as fusions with glutathione-S-transferase. The insert DNA was sequenced to determine the extent of the deletion and the proteins expressed by the clones were examined for the presence of T cell and B cell epitopes. T cell epitopes were mapped by measuring the ability of recombinant antigens to stimulate gamma interferon (gamma-IFN) production in a whole blood culture system. gamma-IFN production was measured using a sandwich enzyme immunoassay sp..

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University of Melbourne Researchers