Journal article

Global metabolic analyses identify key differences in metabolite levels between polymyxin-susceptible and polymyxin-resistant Acinetobacter baumannii

Mohd Hafidz Mahamad Maifiah, Soon-Ee Cheah, Matthew D Johnson, Mei-Ling Han, John D Boyce, Visanu Thamlikitkul, Alan Forrest, Keith S Kaye, Paul Hertzog, Anthony W Purcell, Jiangning Song, Tony Velkov, Darren J Creek, Jian Li

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2016

Abstract

Multidrug-resistant Acinetobacter baumannii presents a global medical crisis and polymyxins are used as the last-line therapy. This study aimed to identify metabolic differences between polymyxin-susceptible and polymyxin-resistant A. baumannii using untargeted metabolomics. The metabolome of each A. baumannii strain was measured using liquid chromatography-mass spectrometry. Multivariate and univariate statistics and pathway analyses were employed to elucidate metabolic differences between the polymyxin-susceptible and -resistant A. baumannii strains. Significant differences were identified between the metabolic profiles of the polymyxin-susceptible and -resistant A. baumannii strains. The ..

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Grants

Awarded by National Institute of Allergy and Infectious Diseases of the National Institutes of Health


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

J.L., D.J.C., T.V., J.S., A.W.P., P.H., A.F. and K.S.K. are supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (R01 AI111965). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Allergy and Infectious Diseases or the National Institutes of Health. J.L. and A.W.P. are Australian NHMRC Senior Research Fellows. D.J.C. and T.V. are Australian NHMRC Career Development Research Fellows. P.H. is an Australian NHMRC Senior Principal Research Fellow.