Journal article
Increased miR-155-5p and reduced miR-148a-3p contribute to the suppression of osteosarcoma cell death
S Bhattacharya, AM Chalk, AJM Ng, TJ Martin, AC Zannettino, LE Purton, J Lu, EK Baker, CR Walkley
Oncogene | NATURE PUBLISHING GROUP | Published : 2016
DOI: 10.1038/onc.2016.68
Abstract
Osteosarcoma (OS) is the most common cancer of bone and the 5th leading cause of cancer-related death in young adults. Currently, 5-year survival rates have plateaued at ∼70% for patients with localized disease. Those with disseminated disease have an ∼20% 5-year survival. An improved understanding of the molecular genetics of OS may yield new approaches to improve outcomes for OS patients. To this end, we applied murine models that replicate human OS to identify and understand dysregulated microRNAs (miRNAs) in OS. miRNA expression patterns were profiled in murine primary osteoblasts, osteoblast cultures and primary OS cell cultures (from primary and paired metastatic locations) isolated fr..
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Funding Acknowledgements
We thank A Pfeiffer (University of Bonn) for providing the miR-155-5p sponge, control sponge and miR-155-5p overexpression vector. We thank A Gupte for technical assistance; C Hawkins for discussion and comments; the BioResource Facility (St Vincent's Hospital) for housing and care of experimental animals, and M Thomson (SVI Flow Cytometry Facility) for help with FACS analysis. This work was supported by grants from the National Health and Medical Research Council (NHMRC), Australia (CW and TJM), Cancer Council of Victoria (CW and EB); Cure Cancer Australia Foundation (EB); 5point foundation (EB); Zig Inge Foundation (CW); NHMRC Career Development Award (CW); NHMRC Dora Lush postgraduate scholarship (SB); in part by the Victorian State Government OIS Program (to St. Vincent's Institute); CW was the Phillip Desbrow Senior Research Fellow of the Leukaemia Foundation.