Journal article

Stability and Reproducibility Underscore Utility of RT-QuIC for Diagnosis of Creutzfeldt-Jakob Disease

Maria Cramm, Matthias Schmitz, Andre Karch, Eva Mitrova, Franziska Kuhn, Bjoern Schroeder, Alex Raeber, Daniela Varges, Yong-Sun Kim, Katsuya Satoh, Steven Collins, Inga Zerr

MOLECULAR NEUROBIOLOGY | SPRINGER | Published : 2016

Abstract

Real-time quaking-induced conversion (RT-QuIC) allows the amplification of miniscule amounts of scrapie prion protein (PrP(Sc)). Recent studies applied the RT-QuIC methodology to cerebrospinal fluid (CSF) for diagnosing human prion diseases. However, to date, there has not been a formal multi-centre assessment of the reproducibility, validity and stability of RT-QuIC in this context, an indispensable step for establishment as a diagnostic test in clinical practice. In the present study, we analysed CSF from 110 prion disease patients and 400 control patients using the RT-QuIC method under various conditions. In addition, "blinded" ring trials between different participating sites were perfor..

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Grants

Awarded by European Commission


Awarded by EU Joint Program-Neurodegenerative Disease Research (JPND-DEMTEST)


Awarded by Robert Koch-Institute


Funding Acknowledgements

This work was supported by a grant from the European Commission (Protecting the food chain from prions: shaping European priorities through basic and applied research (PRIORITY, No. 222887) project number: FP7-KBBE-2007-2A). The study was performed within the recently established Clinical Dementia Center at the University Medical Hospital Gottingen and was partly supported by grants from the EU Joint Program-Neurodegenerative Disease Research (JPND-DEMTEST (Biomarker based diagnosis of rapid progressive dementias-optimization of diagnostic protocols, 01ED1201A) and by the Robert Koch-Institute through funds of the Federal Ministry of Health (grant no. 1369-341).