Determination of Active Phagocytosis of Unopsonized Porphyromonas gingivalis by Macrophages and Neutrophils Using the pH-Sensitive Fluorescent Dye pHrodo
Jason C Lenzo, Neil M O'Brien-Simpson, Jessica Cecil, James A Holden, Eric C Reynolds
INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2016
Phagocytosis of pathogens is an important component of the innate immune system that is responsible for the removal and degradation of bacteria as well as their presentation via the major histocompatibility complexes to the adaptive immune system. The periodontal pathogen Porphyromonas gingivalis exhibits strain heterogeneity, which may affect a phagocyte's ability to recognize and phagocytose the bacterium. In addition, P. gingivalis is reported to avoid phagocytosis by antibody and complement degradation and by invading phagocytic cells. Previous studies examining phagocytosis have been confounded by both the techniques employed and the potential of the bacteria to invade the cells. In thi..View full abstract
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Awarded by Australian Government Department of Industry, Innovation and Science (Cooperative Research Centre)
Awarded by National Health and Medical Research Council
This work was funded by the Australian Government Department of Industry, Innovation and Science (Cooperative Research Centre grant 20080108) and a National Health and Medical Research Council project grant (APP1029878).