Journal article
Evaluation of a conserved HA274-288 epitope to detect antibodies to highly pathogenic avian influenza virus H5N1 in Indonesian commercial poultry
Nadeeka K Wawegama, Simson Tarigan, Risa Indriani, Paul Selleck, RM Abdul Adjid, Tati Syafriati, undefined Hardiman, Peter A Durr, Jagoda Ignjatovic
Avian Pathology | Taylor & Francis | Published : 2016
Abstract
ABSTRACT: A peptide enzyme linked immunosorbent assay (ELISA) based on an epitope in the haemagglutinin (HA) of avian influenza virus H5N1, amino acid positions 274–288 (HA274–288) was evaluated for detection of H5N1-specific antibodies. An optimized ELISA based on the tetrameric form of the HA274–288 epitope designated MP15 gave low background with non-immune chicken sera and detected vaccinated and infected birds. The HA274–288 epitope was highly conserved in Indonesian H5N1 strains and antibody responses were detected in the majority of the vaccinated chickens regardless of the H5N1 strain used for vaccination. The HA274–288 epitope was also conserved in the majority of H5N1 strains from ..
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Awarded by Australian Centre for International Agricultural Research
Funding Acknowledgements
This work was supported by the Australian Centre for International Agricultural Research through projects [AH/2010/039] and [AH/2006/050].