CD11b immunophenotyping identifies inflammatory profiles in the mouse and human lungs

M Duan; DP Steinfort; D Smallwood; M Hew; W Chen; M Ernst; LB Irving; GP Anderson; ML Hibbs

Journal article

CD11b immunophenotyping identifies inflammatory profiles in the mouse and human lungs

M Duan, DP Steinfort, D Smallwood, M Hew, W Chen, M Ernst, LB Irving, GP Anderson, ML Hibbs

Mucosal Immunology | Published : 2016

DOI: 10.1038/mi.2015.84

Abstract

The development of easily accessible tools for human immunophenotyping to classify patients into discrete disease endotypes is advancing personalized therapy. However, no systematic approach has been developed for the study of inflammatory lung diseases with often complex and highly heterogeneous disease etiologies. We have devised an internally standardized flow cytometry approach that can identify parallel inflammatory alveolar macrophage phenotypes in both the mouse and human lungs. In mice, lung innate immune cell alterations during endotoxin challenge, influenza virus infection, and in two genetic models of chronic obstructive lung disease could be segregated based on the presence or ab..

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University of Melbourne Researchers

Daniel Steinfort Author

David Smallwood Author

Louis Irving Author

Gary Anderson Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

We thank Karlheinz Peter for providing CD11b<SUP>-/-</SUP> mice; the respiratory registrars and Michelle Thomson at the Royal Melbourne Hospital for patient consent; Maverick Lau for TUNEL staining protocols; Evelyn Tsantikos for SHIP-1<SUP>-/-</SUP> genotyping; the AMREP flow cytometry facility for support; Stephen Cody and Chad Johnson from Monash Micro Imaging (Monash University) for scientific and technical assistance; and Kylie Spark, Steve Comber; and Naomi Borg from MICU and MARP animal facilities (Monash University) for monitoring the animals. This work was made possible through funding from the NHMRC, Australia; project grants 1008298 and 1080274 (to M.L.H. and G.P.A.) and 1067244 (to M.E.), program grant 1071916 (to W.C.) and support from the Victorian State Government Operational Infrastructure Scheme. M.D. was a recipient of a Cancer Research Institute Scholarship and the Nick Christopher Top-Up Scholarship, while M.E., W.C. and M.L.H. were supported by NHMRC Research Fellowships.