Effect of ovarian hormones on the healthy equine uterus: A global gene expression analysis
CD Marth, ND Young, LY Glenton, DM Noden, GF Browning, N Krekeler
Reproduction Fertility and Development | CSIRO Publishing | Published : 2015
© CSIRO 2016. The physiological changes associated with the varying hormonal environment throughout the oestrous cycle are linked to the different functions the uterus needs to fulfil. The aim of the present study was to generate global gene expression profiles for the equine uterus during oestrus and Day 5 of dioestrus. To achieve this, samples were collected from five horses during oestrus (follicle >35mm in diameter) and dioestrus (5 days after ovulation) and analysed using high-throughput RNA sequencing techniques (RNA-Seq). Differentially expressed genes between the two cycle stages were further investigated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway ..View full abstract
The authors thank Pasi Korhonen, Cristian Alvarez and Ross Hall for the excellent advice and guidance they provided on analysing RNA-Seq data. This work was supported by funds from an Early Career Research Grant to NK provided by The University of Melbourne and the Department of Biomedical Sciences, Cornell University College of Veterinary Medicine (Ithaca, NY, USA).