Journal article

Biasing Amacrine Subtypes in the Atoh7 Lineage through Expression of Barhl2

Patricia R Jusuf, Shahad Albadri, Alessio Paolini, Peter D Currie, Francesco Argenton, Shin-ichi Higashijima, William A Harris, Lucia Poggi



Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor B..

View full abstract

University of Melbourne Researchers


Awarded by Deutsche Forschungsgemeinschaft Research Grant

Awarded by Wellcome Trust Programme Grant

Awarded by Australian National Health and Medical Research Council CJ Martin training fellowship

Funding Acknowledgements

This work was supported by Deutsche Forschungsgemeinschaft Research Grant PO 1440/1-1 to L. P., Wellcome Trust Programme Grant ID RG49253 to W. A. H., and Australian National Health and Medical Research Council CJ Martin training fellowship ID 454798 to P.R.J. S. A. and A. P. are funded by Landesgraduiertenforderung (Funding program of the State of Baden-Wurttemberg). We thank J. L. Mateo Cerdan and D. S. Mosti for advice with statistical analyses; S. Schultz for help with Western blot experiments; and B. Wittbrodt, E. Leist, A. Saraceno, M. Majewski, B. Seiferling, T. Kellner, L. Schertel, C. Mueller, and Julian Cocks for fish maintenance and technical assistance. We are grateful to M. Carl, J. Wittbrodt, L. Centanin, G. Lupo, and M. Zigman for valuable discussion and comments on the manuscript. We also thank J. Wittbrodt for generous support.