Journal article
High resolution FTIR imaging provides automated discrimination and detection of single malaria parasite infected erythrocytes on glass
D Perez-Guaita, D Andrew, P Heraud, J Beeson, D Anderson, J Richards, BR Wood
Faraday Discussions | ROYAL SOC CHEMISTRY | Published : 2016
DOI: 10.1039/c5fd00181a
Abstract
New highly sensitive tools for malaria diagnostics are urgently needed to enable the detection of infection in asymptomatic carriers and patients with low parasitemia. In pursuit of a highly sensitive diagnostic tool that can identify parasite infections at the single cell level, we have been exploring Fourier transform infrared (FTIR) microscopy using a Focal Plane Array (FPA) imaging detector. Here we report for the first time the application of a new optic configuration developed by Agilent that incorporates 25× condenser and objective Cassegrain optics with a high numerical aperture (NA = 0.81) along with additional high magnification optics within the microscope to provide 0.66 micron p..
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Awarded by Australian Research Council
Funding Acknowledgements
Funding to the authors was provided by the Australian Research Council (Future Fellowship FT120100926 to BRW), National Health and Medical Research Council of Australia (Program grant and Senior Research Fellowship to JGB; Early Career Fellowships JSR; Infrastructure for Research Institutes Support Scheme Grant to the Burnet Institute); Victorian State Government Operational Infrastructure Support Grant to the Burnet Institute. We acknowledge Mr Finlay Shanks for instrumental support and Dr Mustafa Kansiz (Agilent Technologies) for loaning us the 25x Cassegrain objectives used for the measurements.