Journal article
Th17 cell differentiation proceeds independently of IRF8
DM Newman, PSK Leung, TL Putoczki, SL Nutt, E Cretney
Immunology and Cell Biology | NATURE PUBLISHING GROUP | Published : 2016
DOI: 10.1038/icb.2016.33
Abstract
The transcriptional repressor/activator interferon regulatory factor 8 (IRF8) modulates the differentiation of a multitude of hematopoietic lineages. However, the role of IRF8 in CD4 + T-cell development is less well defined, with a recent study implicating IRF8 as an intrinsic repressor of interleukin-17 (IL-17) expressing T helper type 17 (Th17) cell differentiation. Using an IRF8-EGFP reporter strain we have confirmed that IRF8 is expressed in all T helper lineages, including Th17 cells. The loss of IRF8 did not affect Th17 differentiation in vitro, beyond a small increase in IL-22 expression. Moreover, IRF8 deficiency did not enhance the Th17 immune response in experimental T-cell transf..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
We thank Herbert Morse III and Hongsheng Wang (NIAID NIH, MD, USA) for the IRF8-EGFP mice, Jamie Leahy (WEHI Bioservices) for technical support and WEHI Flow Cytometry Laboratory staff for assistance in cell sorting. This research was supported by the National Health and Medical Research Council of Australia program Grant No. 1054925 to SLN, project Grant No. 1047313 to EC and fellowships to EC and SLN. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIIS.