Journal article

Ability of the Encephalitic Arbovirus Semliki Forest Virus To Cross the Blood-Brain Barrier Is Determined by the Charge of the E2 Glycoprotein

Mhairi C Ferguson, Sirle Saul, Rennos Fragkoudis, Sabine Weisheit, Jonathan Cox, Adjanie Patabendige, Karen Sherwood, Mick Watson, Andres Merits, John K Fazakerley

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2015

Abstract

UNLABELLED: Semliki Forest virus (SFV) provides a well-characterized model system to study the pathogenesis of virus encephalitis. Several studies have used virus derived from the molecular clone SFV4. SFV4 virus does not have the same phenotype as the closely related L10 or the prototype virus from which its molecular clone was derived. In mice, L10 generates a high-titer plasma viremia, is efficiently neuroinvasive, and produces a fatal panencephalitis, whereas low-dose SFV4 produces a low-titer viremia, rarely enters the brain, and generally is avirulent. To determine the genetic differences responsible, the consensus sequence of L10 was determined and compared to that of SFV4. Of the 12 ..

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Grants

Awarded by Pirbright Institute


Awarded by Estonian Ministry of Education and Research


Awarded by Estonian Science Foundation


Awarded by NERC


Awarded by MRC


Awarded by BBSRC


Awarded by Biotechnology and Biological Sciences Research Council


Awarded by Medical Research Council


Awarded by National Centre for the Replacement


Funding Acknowledgements

This work was supported by Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Project Grants (ISPGs) at The Roslin Institute and The Pirbright Institute (BBB/E/I/00001735). This work was supported by institutional research funding (IUT20-27) from the Estonian Ministry of Education and Research, Estonian Science Foundation (grant 9400), and the European Union through the European Regional Development Fund via the Centre of Excellence in Chemical Biology.Sequencing was carried out by Edinburgh Genomics, The University of Edinburgh. Edinburgh Genomics is partly supported through core grants from NERC (R8/H10/56), MRC (MR/K001744/1), and BBSRC (BB/J004243/1).