Journal article

Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses

Esther Schnettler, Hana Tykalova, Mick Watson, Mayuri Sharma, Mark G Sterken, Darren J Obbard, Samuel H Lewis, Melanie McFarlane, Lesley Bell-Sakyi, Gerald Barry, Sabine Weisheit, Sonja M Best, Richard J Kuhn, Gorben P Pijlman, Margo E Chase-Topping, Ernest A Gould, Libor Grubhoffer, John K Fazakerley, Alain Kohl

NUCLEIC ACIDS RESEARCH | OXFORD UNIV PRESS | Published : 2014

Abstract

Arboviruses are transmitted by distantly related arthropod vectors such as mosquitoes (class Insecta) and ticks (class Arachnida). RNA interference (RNAi) is the major antiviral mechanism in arthropods against arboviruses. Unlike in mosquitoes, tick antiviral RNAi is not understood, although this information is important to compare arbovirus/host interactions in different classes of arbovirus vectos. Using an Ixodes scapularis-derived cell line, key Argonaute proteins involved in RNAi and the response against tick-borne Langat virus (Flaviviridae) replication were identified and phylogenetic relationships characterized. Analysis of small RNAs in infected cells showed the production of virus-..

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Grants

Awarded by Netherlands Organisation for Scientific Research NWO [Rubicon fellowship]


Awarded by Wellcome Trust [Biomedical Resources Grant]


Awarded by FP7-PEOPLE-ITN programme [EU Grant]


Awarded by Czech Science Foundation (GACR)


Awarded by National Institutes of Health


Awarded by Biotechnology and Biological Sciences Research Council


Awarded by Medical Research Council


Awarded by Natural Environment Research Council


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Awarded by BBSRC


Awarded by MRC


Funding Acknowledgements

Netherlands Organisation for Scientific Research NWO [Rubicon fellowship, 825.10.021 to E. S.]; UK Biotechnology and Biological Sciences Research Council [Roslin Institute Strategic Programme Grant to J.K.F. and A. K.]; UK Medical Research Council [to A. K. and E. S.]; Wellcome Trust [Biomedical Resources Grant 088588 to J.K.F. and L. B. S., RCDF 085064/Z/08/Z to D.O.]; FP7-PEOPLE-ITN programme [EU Grant No. 238511 POSTICK ITN to S. W.]; Czech Science Foundation (GACR) [P302/12/2490 to H. T.], National Institutes of Health [AIO055672 to R.J.K.]; Division of Intramural Research, National Institutes of Health, National Institute of Allergy and Infectious Diseases [to S. M. B.]. Funding for open access charge: UK Medical Research Council.