Journal article

Retinal Changes in an ATP-Induced Model of Retinal Degeneration

Felix P Aplin, Kirstan A Vessey, Chi D Luu, Robyn H Guymer, Robert K Shepherd, Erica L Fletcher

FRONTIERS IN NEUROANATOMY | FRONTIERS MEDIA SA | Published : 2016

Abstract

In rodents and felines, intravitreal administration of adenosine triphosphate (ATP) has been shown to induce photoreceptor death providing a tractable model of retinal degeneration in these species. This study investigated the long term effects of photoreceptor loss in an ATP induced feline model of retinal degeneration. Six normal sighted felines were unilaterally blinded using intravitreal ATP injections and assessed using electroretinography (ERG) and optical coherence tomography (OCT). At 30 h (n = 3) or 12 weeks (n = 3) post-injection, the animals were euthanized and the eyes enucleated. Retinae were sectioned and labeled using immunohistochemistry for markers of cell death, neural remo..

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