Retinal Changes in an ATP-Induced Model of Retinal Degeneration

Felix P Aplin; Kirstan A Vessey; Chi D Luu; Robyn H Guymer; Robert K Shepherd; Erica L Fletcher

Journal article

Retinal Changes in an ATP-Induced Model of Retinal Degeneration

Felix P Aplin, Kirstan A Vessey, Chi D Luu, Robyn H Guymer, Robert K Shepherd, Erica L Fletcher

FRONTIERS IN NEUROANATOMY | FRONTIERS MEDIA SA | Published : 2016

DOI: 10.3389/fnana.2016.00046

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Abstract

In rodents and felines, intravitreal administration of adenosine triphosphate (ATP) has been shown to induce photoreceptor death providing a tractable model of retinal degeneration in these species. This study investigated the long term effects of photoreceptor loss in an ATP induced feline model of retinal degeneration. Six normal sighted felines were unilaterally blinded using intravitreal ATP injections and assessed using electroretinography (ERG) and optical coherence tomography (OCT). At 30 h (n = 3) or 12 weeks (n = 3) post-injection, the animals were euthanized and the eyes enucleated. Retinae were sectioned and labeled using immunohistochemistry for markers of cell death, neural remo..

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University of Melbourne Researchers

Robert Shepherd Author Medical Bionics Department

Chi Luu Author Ophthalmology Eye and Ear Hospital

Erica Fletcher Author Anatomy and Physiology

Kirstan Vessey Author Anatomy and Physiology

Robyn Guymer Author Ophthalmology Eye and Ear Hospital

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Grants

Awarded by National Health and Medical Research Counci (NHMRC)

Funding Acknowledgements

This work was supported by the Australian Research Council (ARC) through its Special Research Initiative (SRI) in Bionic Vision Science and Technology grant to Bionic Vision Australia. (BVA) and by the National Health and Medical Research Counci (NHMRC) project grant (APP10210412; APP1061419) to ELF by Retina Australia. The Bionics Institute and CERA receive Operational Infrastructure Support from the Victorian Government.