Conventional protein kinase C beta-mediated phosphorylation inhibits collapsin response-mediated protein 2 proteolysis and alleviates ischemic injury in cultured cortical neurons and ischemic stroke-induced mice
Xuan Yang, Xinxin Zhang, Yun Li, Song Han, David W Howells, Shujuan Li, Junfa Li
JOURNAL OF NEUROCHEMISTRY | WILEY | Published : 2016
We previously reported that conventional protein kinase C (cPKC)β participated in hypoxic preconditioning-induced neuroprotection against cerebral ischemic injury, and collapsin response-mediated protein 2 (CRMP2) was identified as a cPKCβ interacting protein. In this study, we explored the regulation of CRMP2 phosphorylation and proteolysis by cPKCβ, and their role in ischemic injury of oxygen-glucose deprivation (OGD)-treated cortical neurons and brains of mice with middle cerebral artery occlusion-induced ischemic stroke. The results demonstrated that cPKCβ-mediated CRMP2 phosphorylation via the cPKCβ-selective activator 12-deoxyphorbol 13-phenylacetate 20-acetate (DOPPA) and inhibition o..View full abstract
Awarded by National Natural Science Foundation of China
Awarded by Beijing Natural Science Foundation
Awarded by Seed Grant of International Alliance of Translational Neuroscience
This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 31171147 and 81301015), the Beijing Natural Science Foundation (Grant Nos. 7132070 and 7141001), and the Seed Grant of International Alliance of Translational Neuroscience (PXM2014-014226-000006). The authors confirm that there are no conflicts.