Journal article

SIGNAL-TRANSDUCTION BY THE GROWTH-HORMONE RECEPTOR

MJ WATERS, SW ROWLINSON, RW CLARKSON, CM CHEN, PE LOBIE, G NORSTEDT, H MERTANI, G MOREL, R BRINKWORTH, CA WELLS, S BASTIRAS, AR ROBINS, GE MUSCAT, RT BARNARD

PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE | WILEY-BLACKWELL | Published : 1994

Abstract

It has been proposed that dimerization of identical receptor subunits by growth hormone (GH) is the mechanism of signal transduction across the cell membrane. We present here data with analogs of porcine GH (pGH), with GH receptors (GHR) mutated in the dimerization domain and with monoclonal antibodies to the GHR which indicate that dimerization is necessary but not sufficient for transduction. We also report nuclear uptake of GH both in vivo and in vitro, along with nuclear localization of the receptor and GH-binding protein (GHBP). This suggests that GH acts directly at the nucleus, and one possible target for this action is a rapid increase in transcription of C/EBP delta seen in 3T3-F442..

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University of Melbourne Researchers