Selection of Reference Genes for Gene Expression Studies related to lung injury in a preterm lamb model
Prue M Pereira-Fantini, Anushi E Rajapaksa, Regina Oakley, David G Tingay
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2016
Preterm newborns often require invasive support, however even brief periods of supported ventilation applied inappropriately to the lung can cause injury. Real-time quantitative reverse transcriptase-PCR (qPCR) has been extensively employed in studies of ventilation-induced lung injury with the reference gene 18S ribosomal RNA (18S RNA) most commonly employed as the internal control reference gene. Whilst the results of these studies depend on the stability of the reference gene employed, the use of 18S RNA has not been validated. In this study the expression profile of five candidate reference genes (18S RNA, ACTB, GAPDH, TOP1 and RPS29) in two geographical locations, was evaluated by dedic..View full abstract
Awarded by National Health and Medical Research Council Project Grant, Canberra, Australia
Awarded by National Health and Medical Research Council
This study is supported by a National Health and Medical Research Council Project Grant, Canberra, Australia (Grant ID 1009287) and the Victorian Government Operational Infrastructure Support Program (Melbourne, Australia). D.G.T. is supported by a National Health and Medical Research Council Clinical Career Development Fellowship (Grant ID 1053889).