Journal article

Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia

Brian Dean, Madhara Udawela, Elizabeth Scarr

BMC PSYCHIATRY | BIOMED CENTRAL LTD | Published : 2016

Abstract

BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug treatments. The complexity of human CNS means it unlikely that any gene could fulfil these criteria. METHODS: To address this issue we measured levels of mRNA for six potential reference genes (GAPDH, PPIA, SNCA, NOL9, TFB1M and SKP1) in three cortical regions (Brodmann's areas (BA) 8, 9 and 44) from 30 subjects with schizophrenia and 30 age and sex matched controls. We used a structured statistical approach to examine the characteristics of these..

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Grants

Awarded by NHMRC Fellowship


Awarded by Australian Research Council


Awarded by NHMRC Project Grant


Funding Acknowledgements

This research was funded in part by the CRC for Mental Health, NHMRC Project Grant APP1045619 and the Victorian Government's Operational Infrastructure Support. BD is the recipient of an NHMRC Fellowship (APP1002240). ES was supported by the Australian Research Council (Future Fellowship FT100100689). The Victorian Brain Bank Network is supported by the Florey Institute for Neuroscience and Mental Health, the Alfred Hospital, the Victorian Forensic Institute of Medicine, the University of Melbourne and funded by Australia's National Health & Medical Research Council, Helen Macpherson Smith Trust, Parkinson's Victoria and Perpetual Philanthropic Services.