Journal article
Identification of ovarian cancer-associated proteins in symptomatic women: A novel method for semi-quantitative plasma proteomics
KL Shield-Artin, MJ Bailey, K Oliva, AK Liovic, G Barker, NL Dellios, S Reisman, M Ayhan, GE Rice
Proteomics Clinical Applications | WILEY-V C H VERLAG GMBH | Published : 2012
Abstract
Purpose: To evaluate the utility of an enhanced biomarker discovery approach in order to identify potential biomarkers relevant to ovarian cancer detection. Experimental design: We combined immuno-depletion, liquid-phase IEF, 1D-DIGE, MALDI-TOF/MS and LC-MS/MS to identify differentially expressed proteins in the plasma of symptomatic ovarian cancer patients, stratified by stage, compared to samples obtained from normal subjects. Results: We demonstrate that this approach is a practical alternative to traditional 2D gel techniques and that it has some advantages, most notably increased protein capacity. Proteins were identified in all 76 bands excised from the gels in this project and confirm..
View full abstractGrants
Awarded by Australian NHMRC
Funding Acknowledgements
G.E.R. was in receipt of an NHMRC Principal Research Fellowship. The work detailed herein was, in part, funded by the Fight Cancer Foundation, Lion Nathan, BHP Billiton and an equipment grant from the Harold and Corra Brennen Benevolent Trust. Proteomic data analysis described in this work was supported by the use of the Australian Proteomics Computational Facility funded by the Australian NH&MRC under grant No. 381413.