Cryptosporidium and Giardia taxa in faecal samples from animals in catchments supplying the city of Melbourne with drinking water (2011 to 2015)
Anson V Koehler, Shane R Haydon, Aaron R Jex, Robin B Gasser
PARASITES & VECTORS | BMC | Published : 2016
BACKGROUND: In a long-term program to monitor pathogens in water catchments serving the City of Melbourne in the State of Victoria in Australia, we detected and genetically characterised Cryptosporidium and Giardia in faecal samples from various animals in nine water reservoir areas over a period of 4 years (July 2011 to November 2015). METHODS: This work was conducted using PCR-based single-strand conformation polymorphism (SSCP) and phylogenetic analyses of portions of the small subunit of ribosomal RNA (SSU) and 60 kDa glycoprotein (gp60) genes for Cryptosporidium, and triose-phosphate isomerase (tpi) gene for Giardia. RESULTS: The prevalence of Cryptosporidium was 1.62 % (69 of 4,256 sam..View full abstract
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Awarded by Australian Research Council
Funding from the Australian Research Council (grant no. LP130100209) and Melbourne Water is gratefully acknowledged, as is support from the National Health and Medical Research Council (NHMRC) of Australia.