Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayed death in Plasmodium falciparum
Charisse Flerida A Pasaje, Vanessa Cheung, Kit Kennedy, Erin E Lim, Jonathan B Baell, Michael DW Griffin, Stuart A Ralph
Scientific Reports | NATURE PUBLISHING GROUP | Published : 2016
Awarded by Australian National Health and Medical Research Council
Awarded by Australian Research Council
Awarded by NHMRC RD Wright Biomedical fellowship
This work was supported by an Australian National Health and Medical Research Council grant (628704). M.D.W.G is a recipient of the C.R. Roper Fellowship and an Australian Research Council Future Fellowship (project number FT140100544). S.A.R is supported by an NHMRC RD Wright Biomedical fellowship (APP1062504). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. We thank Geoffrey McFadden at the University of Melbourne for the ACP-DsRed/CS-eYFP 3D7 P. falciparum. The E. coli mutant KY4040 was obtained from The E. coli Genetic Stock Center at Yale University. We thank Wim G. Hol at the SSGCID and Wes Van Voorhis at the CERID for providing the cytosolic TrpRS plasmid used in determining enzyme kinetics. The Australian Red Cross Blood Service kindly supplied blood.